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Green fluorescence filter sets (Leitz) with excitation wavelength of 480±20 nm and emission wavelength of 510 nm were used with the same microscope for visualisation of FITC and Alexa Fluor 488.
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Fresh transferrin solutions of Fe2-Tf (sample 3, 12.5 μ m) were prepared in 24 m m sodium bicarbonate buffer (mimicking blood concentration), and a 20 μL aliquot of the sample was spread onto a glass coverslip and allowed to dry at room temperature for visualisation under an optical microscope.
For visualisation and acquisition of images, an Axio Imager Z1 (ZEISS) epifluorescence microscope equipped with a CoolSnap CCD camera was used.
Epifluorescence images were taken using a Nikon-Ti Eclipse microscope with a 60X (1.4 NA) oil-immersion objective, DS-Qi1Mc candra and standard filter sets for visualisation of DAPI, GFP and Alexa568.
For visualisation of the black-blue stained fat granules we used a Leica DM IRB microscope (Leica, Wetzlar, Germany) with a digital camera.
js: a graph theory library for visualisation and analysis.
Note that resultant gnuplot scripts resulting from mummerplot were edited for visualisation.
Worker types and sample output data for visualisation.
Twenty-five meV Gaussian smearing applied for visualisation purposes.
This makes them accessible for visualisation with CT [15].
Slides were stained with DAPI for visualisation of kinetoplast and nuclear DNA content.
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