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The sections were washed, mounted onto glass slides, coverslipped, and observed under the confocal microscope for image acquisition and initial processing.
Samples were observed on a Zeiss LSM 510 laser scanning confocal microscope for image acquisition.
Briefly, paraformaldehyde fixed cells processed by immunofluorescence were examined on a Zeiss LSM 510 laser scanning confocal microscope for image acquisition.
Fluorescence was observed on a Carl Zeiss LSM 700 confocal laser-scanning microscope for image acquisition.
Image analyses were performed using a Nikon DXM 1200 digital camera (Tokyo, Japan) mounted onto an Eclipse Nikon E-800 microscope for image acquisition.
Image analysis measurements were made using a KS400 system (Kontron Elektronic/Zeiss, Eching, Germany) and a Nikon DXM 1200 digital camera (Tokyo, Japan) mounted onto an Eclipse Nikon E-800 microscope for image acquisition.
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The microscope used for image capture had been previously calibrated with a stage-micrometer and a conversion factor of 0.135 µm pixel−1 was applied to images captured using the 100× objective.
The high molecular weight DNA was then immobilized as individual molecules onto Optical Chips, digested with EagI or AflII restriction enzymes (New England Biolabs), fluorescently stained with YOYO-1 (Invitrogen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement, resulting in high resolution single-molecule restriction maps.
Briefly, high molecular weight DNA was immobilized as individual molecules onto optical chips, digested with NcoI (New England Biolabs, Ipswich, MA, USA), fluorescently stained with YOYO-1 (Invitrogen, Carlsbad, CA, USA) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement to give high-resolution single-molecule restriction maps.
Select any microscope for automated image acquisition.
Instabilities and limited coherence of the electron beam cause falloff of the signal transfer by the microscope for fine image details, leading to blurring of small features.
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