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It is an old green German microscope, fitted with special lenses that display the transparent interior surfaces of a worm as if they were a bas relief.
Images were taken using a standard fluorescent microscope fitted with an automated quantitative pathology system (Vectra) in conjunction with Vectra 3.3 and inForm 2.3.0 software (Perkin Elmer).
Individual coverslips were transferred to a recording chamber fixed to the stage of the microscope fitted with a × 40 water-immersion lens.
The set-up consists of an inverted optical microscope, fitted with an adapted stage on which two micromanipulators are employed to hold a pair of micropipettes.
Laser focusing on the sample was performed through a petrographic microscope fitted with a 100× objective.
t-AFM measurements were carried out with Veeco DI3100 atomic force microscope fitted with Nanoscope V controller.
Following paraffin-embedding, 5-μm sections were cut and the sections viewed using an Olympus BX41 fluorescence microscope fitted with a ×40 FITC filter.
Fluorescence was analyzed using a Nikon Eclipse microscope fitted with a charge-coupled-device camera.
Histological sections were visualised with a Zeiss Axioplan2.0 microscope fitted with an AxioCam HD camera.
Images were captured on a Leica DMRA2 microscope fitted with a Leica DFC320 camera.
Section images were taken with an Olympus BX51 microscope fitted with DP70 camera (Olympus, Japan).
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