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For quantitative analysis, Brn3a-positive cells were counted in four to six microscope fields of identical size located at approximately the same distance from the optic disc.
Confocal images were taken within a 4 mm×4 mm area defined by microscope fields of view chosen as representative for each density group (40× objective, SP2 laser scanning confocal microscope, Leica Microsystems, Mannheim, Germany).
Digital images were captured of non-overlapping, consecutive microscope fields of the myelinated cultures.
Cells in five different microscope fields of each Ki-67 staining tissue were used to calculate positive cells.
Borrelia spp. spirochetes were identified by observing 200 microscope fields of Giemsa-stained thin blood smears under an oil-immersion objective (magnification ×1,000).
Automated tiling of adjacent microscope fields of view was completed to generate images of an entire tumor section at a resolution of 0.75 μm per pixel.
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Chains longer than the length of the microscope field of imaging were obtained at r = 24.
Strong magnetic field gradients, produced by the wires, exert sufficient magnetic forces on the mRBCs in order to separate and concentrate them in a specific region small enough to fit within the microscope field of view at magnifications typically required to identify the malaria parasite type.
Due to heterogeneity within the tumor microenvironment both within different regions of the same tumor (intra-tumoral) and across different tumors within one or more animals (inter-tumoral), it is recommended to maintain the same microenvironment structures (e.g., microvasculature, tissue, and cells) within the microscope field of view for the duration of the experiment.
All measured parameters are reported as the mean of obtained video clips in order to compensate for changes in microscope field of view in between measurements in compliance with the international consensus [32]; however, two instead of three post-intervention video clips for the ACH and NG measurements were acquired in order to limit total examination time.
(B ) Image sequence of the same microscope field of view taken at each of the three dye wavelengths (rows) at five selected time points (t ; columns).
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