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Cells exhibiting a nuclear DAB signal were counted in each of three 1.0-mm-square confocal microscope fields, in each of three spaced coronal sections through the neostriatum in both hemispheres of 6 mice from each treatment group.
To measure the area of medium spiny striatal neurons, images were captured from three 1.0-mm-square confocal microscope fields, in each of three spaced coronal sections in each hemisphere of 6 mice from each group.
The intensity of pCREB staining was calculated in each of three 1.0-mm-square confocal microscope fields, in each of three rostrocaudally spaced sections on each hemisphere from 6 mice from each saline, TP10 treated R6/2 mice and wild type littermates.
Cells were quantified as the number of cells found in five random microscope fields in two independent inserts.
Colour image montages from 16 adjacent microscope fields in each of four tumour regions (encompassing roughly 15 mm) were automatically acquired and digitally combined under three different staining conditions.
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To this aim we sought out couplets of NCM460 cells in the microscope field in which one of the cells expressed the cAMP sponge construct and the other did not (control cell).
This software allows measurements to be made from user-defined areas of the microscope field; in this case, the fluorescence measurements were localized to selected areas of the muscle fibres.
The analysis included the apical, the middle, and the basal turn, with three random microscope fields counted in each explant per cochlear turn.
All measured parameters are reported as the mean of obtained video clips in order to compensate for changes in microscope field of view in between measurements in compliance with the international consensus [32]; however, two instead of three post-intervention video clips for the ACH and NG measurements were acquired in order to limit total examination time.
Nuclei (from mononucleated and multinucleated cells) were counted in fifty randomly chosen microscope fields (3 culture dishes, 50 fields in each dish) at a magnification of x 400.
The number of myofibrils present within myotubes was counted in fifty randomly chosen microscope fields (3 culture dishes, 50 fields in each dish) at a magnification of x 400.
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