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CD3 and CD8 cells were counted in six 400× microscope fields and expressed as average percentage (CD8+/CD3+ divided by total CD3) from four cases.
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Mean intensity of green fluorescence in cells in three microscope fields per well and three wells for each condition was used to determine average Htt levels.
Six random high-power (100x) microscope fields were examined and the average of these six fields was taken.
Oocysts or cysts were then quantified by counting total numbers in 150 microscope fields (400× magnification) and extrapolating results to the entire sample.
Sections with single label immunostaining for CD45 isoforms (RA, RC, RB and RO) were analyzed by counting the total positive cells in the cerebral white matter of ten 400× random microscope fields per case and averaging the number.
After 12 hours of incubation, six random high-power (100x) microscope fields were examined and graded by two investigators as follows: 0, separated individual cells; 1, cells begin to migrate and align; 2, capillary tubes visible, but no sprouting; 3, sprouting of new capillary tubes visible; 4, closed polygons begin to form; and 5, complex mesh-like structures develop.
Approximately 100 cells from several microscope fields (5 6) were counted and identified for each sample.
Colour images from the same 16 adjacent microscope fields were automatically acquired and digitally combined under four different staining conditions, using a Prior computer interfaced stage and Prior controller to revisit the same stage co-ordinates.
Fluorescence microscopy coupled with compatible filters – which are also available in a multi-well format – enables the detection of small numbers of cells using a low power objective lens and larger microscope fields [36], [37].
Three microscope fields were selected at random and photographed.
Only the cells that migrated within the microscope field were selected and tracked using the "Manual Tracking" plug-in in NIH ImageJ.
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