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Due to heterogeneity within the tumor microenvironment both within different regions of the same tumor (intra-tumoral) and across different tumors within one or more animals (inter-tumoral), it is recommended to maintain the same microenvironment structures (e.g., microvasculature, tissue, and cells) within the microscope field of view for the duration of the experiment.
All measured parameters are reported as the mean of obtained video clips in order to compensate for changes in microscope field of view in between measurements in compliance with the international consensus [32]; however, two instead of three post-intervention video clips for the ACH and NG measurements were acquired in order to limit total examination time.
Strong magnetic field gradients, produced by the wires, exert sufficient magnetic forces on the mRBCs in order to separate and concentrate them in a specific region small enough to fit within the microscope field of view at magnifications typically required to identify the malaria parasite type.
The numbers of fluorescent nuclei were counted in a microscope field of view at ×40 magnification and under an excitation filter I2: BP 450 490.
Scattering spectra of individual nanorods were collected by imaging the microscope field of view to a line-imaging spectrometer (Acton Research SpectraPro 2150i).
(B ) Image sequence of the same microscope field of view taken at each of the three dye wavelengths (rows) at five selected time points (t ; columns).
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For quantitative analysis, Brn3a-positive cells were counted in four to six microscope fields of identical size located at approximately the same distance from the optic disc.
Confocal images were taken within a 4 mm×4 mm area defined by microscope fields of view chosen as representative for each density group (40× objective, SP2 laser scanning confocal microscope, Leica Microsystems, Mannheim, Germany).
Digital images were captured of non-overlapping, consecutive microscope fields of the myelinated cultures.
Cells in five different microscope fields of each Ki-67 staining tissue were used to calculate positive cells.
Automated tiling of adjacent microscope fields of view was completed to generate images of an entire tumor section at a resolution of 0.75 μm per pixel.
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