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In the days when crystals were mounted in thin glass capillaries, these would be fixed to the apex of the goniometer with a blob of plasticene (yes, I know) and the crystal position, monitored though a microscope eyepiece, adjusted up and down, side to side and forwards and backwards by a turn of the key on the square-headed control screws.
The imaging system consisted of a 20X wide field microscope eyepiece (Model NT39-696, Edmundseparated separated by 160 mm from a microscope objective (60X 0.85NA DIN Achromat objective, 160 mm tube length, Model NT38-340, Edmunds Optics).
For this study, we used a 0.85 NA 60X Achromat objective and a 20X wide field microscope eyepiece, resulting in a system field-of-view of ∼180 µm diameter, an effective magnification onto the camera face of ∼28X, and a measured spatial resolution of ∼1.2 µm.
3. Hold the camera lens against the microscope eyepiece.
A graduated calibrated microscope eyepiece graticule (Pyser-SGI, UK) was used to measure TS diameter.
IHC scoring was undertaken by using a microscope eyepiece grid to standardize the assessed area.
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Because the SD image has low quality or resolution, we have evaluate just comparing directly watching microscope eyepieces (depend on optical system) with our proposed system.
Many problems of using a microscope relate to the fact that the surgeon's eyes must remain fixed on the microscope eyepieces.
Looking through microscope eyepieces, while performing a complex microsurgical reconstruction for hours sitting or standing relatively motionless often in uncomfortable positions, is not uncommon.
As an alternative to the operating microscope, advances in video technology can now permit the surgeon to view a microsurgical field on a video monitor in three dimensions without the necessity of physically looking through the microscope eyepieces.
The system uses standard, inexpensive microscope eyepieces and objectives; magnification and resolution can be adjusted by using different objectives.
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