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Vodafone's mission here is to "address critical social issues around the world", mentioning mobile disaster warnings and "a microscope embedded on a mobile phone and used in remote areas to collect and submit blood samples for diagnosis at a faraway lab" as ideas of the right mindset.
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In brief, 4 × 10 cells were mixed with 60 μl of 1% low melting point agarose in PBS (37°C) and embedded on an agarose pre-treated standard microscope slide.
Figure 2 shows the trails produced by nm-size AuNPs (5, 20 and 40 nm) embedded on a collagen fiber matrix during a single acquisition frame when using a raster scanning two-photon microscope.
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Sections were mounted on Superfrost-Plus microscope slides (Allegiance) and stained with toluidine blue for routine study, or were utilized for immunohistochemistry. Collagen sponges were bisected and the two halves embedded on edge.
Tissues for electron microscopy were selected under a dissecting microscope and embedded in araldite.
Globes were embedded sagitally; ONs and chiasmata were embedded longitudinally.
Otoliths were positioned horizontally on a microscope slide and embedded in thermosetting glue (Crystalbond, Aremco Products , Inc , USA
Sections were mounted on glass slides and stained with Safranin and Astra Blue (Luque et al. 1996) and then inspected and photographed using a Leica DM LB2 light microscope with an embedded ICC50 digital camera (Leica Microsystems CMS GmbH, Germany).
One set of digestion experiments was carried out: n = 3 tissue core sections were mounted on microscope slides and hydrogel discs (trypsin embedded) were placed on top and incubated via conventional oven at 50 °C for 4 h.
Single or groups of dendrites or cell fragments were excised from the microscope slide, re-embedded, trimmed and re-sectioned on an ultramicrotome (Fig. 2c j) in series of ultrathin sections using a block advance of 50 nm.
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