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b shows image of fluorescent microscope due to point-contacts before pipetting Epicenter-solution.
Lignin can be localised under a fluorescence microscope, due to autofluorescence of lignin in the green excitation range.
The nanowire could be observed through the template using an optical microscope, due to the transparency of the template.
Those two microscopes have much higher resolutions of tens of nanometers compared to the typical optical microscope due to the shorter de Broglie wavelength of electrons.
It is difficult to observe hydrogel villi structures under the bright field imaging using a conventional microscope due to its bright color and transparency.
For the quantity of cells adhered to the materials could not be observed by optical microscope due to the poor light-admitting quality of GO-chitosan films, the results were measured by tracing the nucleus of cells with DAPI.
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Although relatively simple changes are made to a reflectance confocal microscope to achieve depth encoding, the same changes cannot be applied to one-photon fluorescence confocal microscopes due to the Stokes shift of the fluorescence emission.
However, given the larger dimension (section ∼200 μm) of a mammalian nucleus, this method requires high laser power, not practical with standard microscopes or live cell microscopy due to phototoxicity effects.
This green emittance under fluorescence microscope was due to passage of acridine orange (AO) dye through cell membrane and uptake of AO by internal cellular organelles.
25, 26 These images are then convolved with a 3-D model to account for the spreading of light through the microscope lens due to diffraction, as described below.
Under the fluorescence microscope, fluorescence due to PpIX was seen homogeneously down to the deep portion of HCC, suggesting that PDT with ALA for HCC may be performed efficiently.
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