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No significant correlation between D (µm2/s) values and the expression level of Httex1-GFP (as measured by microscope detector gain) was observed (Fig. 3C).
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We think it unlikely that this lack of detection is caused by a loss of sensitivity by the confocal microscope detectors in the inner layers of the roots, because both propidium iodide staining and YFP signal are easily detected in the xylem and xylem pole pericycle, respectively (Fig. 2 and Additional file 3).
Future developments in microscopes, detectors and montaging of 3D image stacks will resolve this issue and allow us to image the subcellular details of anastomosis.
The instruments needed for PICA include a microscope, a detector that can count photons and a computer to handle the data.
Both microscope and detector were controlled by the SerialEM software package [14] which managed the automated tilt series acquisition.
Using the IR microscope MCT detector, single-cell spectra were acquired using SRIR for both CHO-K1 and DLD1 cells on different IR optical substrates.
Furthermore, in the confocal microscope the detector pinhole rejects light in order to achieve axial sectioning, and in practice, especially in the ophthalmic imaging case of the confocal Scanning Laser Ophthalmoscope (SLO), trade offs have to be made between pinhole size and confocality, thus limiting the axial sectioning capabilities of the device.
The resulting CNTs were characterized by scanning electron microscopy (SEM) (Philips, MAG 15 kV, ×30,000, SE detector microscope, (Philips Tecnai) FEI Co., Hillsboro, OR, USA) and transmission electron microscopy (TEM) (CM10, 100 kV, Philips).
Micro-Fourier transform infrared spectroscopy (FTIR) was performed in a Nicolet Nexus spectrophotometer coupled to a Nicolet Continuum microscope with MCT detector cooled by liquid nitrogen; the spectra were collected in transmittance mode with interval of 4000 650 cm−1, obtaining a resolution of 4 cm−1 and 128 wipes in areas of 50 100 μm with recourse to a Thermo diamond cell of compression.
The resulting CNTs and Ag/CNT nanocomposite were characterized by analyzing the scanned electron microscopy images (SEM Philipss, MAG 15 kV, 30000X, SE detector microscope, FEI Co., Hillsboro, OR, USA) and Fourier transform infrared (FT-IR) spectra (Shimadzu 8400 s, Shimadzu Corporation, Kyoto, Japan).
The tracks density, ρ x, mentioned in Eq. 1 can be obtained from the geometric mean values, µg, so that Eq. 1 becomes: C x = 150 ± 12 μ g / A t x, (3)where A is the area of the viewed surface by the microscope on the detector.
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