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Sex was determined using morphology of the posterior abdominal tergite or the presence/absence of a sclerotized sipho seen with an underlighted microscope; criteria were verified by genital dissection.
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Apoptotic morphology was assessed at 40× and 100× objective on a light microscope using standard criteria.
After 14 days of culture, the number of colony-forming units was evaluated under an inverted microscope according to standard criteria [ 20- 22].
The types of colonies were classified under inverted microscope (Ziess) by morphologic criteria; individual colonies were plucked and further evaluated by Y-chromosome PCR.
The numbers of colonies were counted and types of colonies classified under inverted microscope (Ziess) by morphologic criteria 14 days after plating.
Erythroid colonies (BFU-Es), granulopoietic colonies (CFU-GMs) and multi-lineage colonies (CFU-GEMM) were scored by direct visualization using an inverted microscope (32×) 14 days later using standard criteria [38].
After 2 weeks of culture, the dishes were fixed with ethanol and stained with Geimsa solution (Merck), observed under a dissecting microscope, and judged according to standard criteria [49].
In addition, Rhododendron leaf extract-treated and MitoTracker® Red CMXRos-stained cells were inspected under a fluorescence microscope in accordance with morphological criteria that allow for the determination of the shape of mitochondria (Fig. 4, Additional file 3: Figure S3).
It wasn't medical error: observed under the microscope, the questionable nodules met the criteria for thyroid cancer.
The scaling properties of the microscope as well as the sampling criteria and reliability of the system are discussed in detail.
Bronchoalveolar lavage (BAL) cell differential counts were determined using morphologic criteria under a light microscope with evaluation of ≥ 500 cells/slide.
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