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Following fixation, cells were washed in PBS, adhered to poly-lysine coverslips, and imaged using an Olympus IX-71 fluorescence microscope coupled with a Photometrix CoolSnapHQ CCD (charge-coupled device) camera driven by Delta Vision software (Applied Precision), and images were deconvolved when indicated in the figure legends.
Microscopic observation of tissues was performed with an Olympus Microphot-CX41 microscope coupled with a digital camera.
Microscopic observation of tissues was performed using an Olympus Microphot-CX41 microscope coupled with a digital camera.
Microscopic observation of tissues was carried out with an Olympus Microphot-CX41 microscope coupled with a digital camera.
Particle size was determined by optical microscopy using optical trianocular microscope coupled with a camera at 100× objective.
SEM and energy dispersive X-ray spectroscopy (EDX) analysis were recorded using HITACHI SU6600 microscope coupled with EDX detector.
This outer skin was analysed using a scanning electron microscope coupled with an energy dispersive X-ray spectrometer.
Some fallen leaves from the tested materials were put under a scanning electron microscope coupled with energy dispersive X-ray spectroscopy (SEM/EDX).
Scanning electron microscope coupled with energy dispersive X-ray spectroscopy analysis revealed that the surface structure and elemental components of Lentinan significantly changed after selenizing.
The morphologies of biocomposite were investigated by means of atomic force microscope and transmission electron microscope coupled with energy dispersive spectrum.
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X-ray diffractogram (XRD), Fourier-transform infrared (FTIR) spectra, and scanning-electron-microscope coupled with energy-dispersive-spectroscopy (SEM + EDS) showed that strong alkali (10 M-NaOHaq) influenced the microstructural-density, reduced carbonation and improved binder amorphousity than the mild alkali (4 M-NaOHaq).
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