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To confirm these mat samples were active, they were examined by phase contrast and epifluorescence microscopy with an Olympus BX60 microscope (Center Valley, PA).
Images were obtained using an Olympus Provis AX70 microscope (Center Valley, PA) equipped with a digital camera and processed using Image-Pro Plus (Media Cybernetics, Silver Spring, MD).
Fluorescence imaging was performed on an Olympus IX71 X-Cite 120 fluorescence microscope (Center Valley, PA) followed by quantitation using Image J. RNA was isolated from primary airway cultures greater than 2 weeks old or primary lung tissue using TRIzol with the Pure Link RNA kit (Invitrogen).
Images were captured on an Olympus BX50 light microscope (Center Valley, PA USA) at 20 × magnification.
Cells were imaged on an Olympus Fluoview 1000 confocal microscope (Center Valley, PA).
Sections were visualized using Olympus IX81 light microscope (Center Valley, PA).
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Microscopic images were obtained using an Olympus FluoView 500 Laser Scanning Confocal Microscope (Olympus, Center Valley, PA, USA) equipped with a 20× objective.
High-resolution transmission electron microscope image (center) revealing the single-crystalline nature of the crystal.
Immunohistochemistry was analyzed using an inverted light microscope (Olympus, Center Valley, PA).
Confocal laser scanning was performed with a FV500 confocal laser-scanning microscope (Olympus, Center Valley, PA).
DIC and epifluorescence images were taken using Olympus IX81 inverted microscope (Olympus, Center Valley, PA).
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