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Volume percentages, accurate to within 1 percent, can be estimated under the microscope by using a point-counting technique over a plane section of the rock; volumes also can be approximated visually in hand specimens in the field.
Photos were taken under Olympus BX51 microscope by using a bright-field illumination.
The microstructure associated with the hydrogen-induced features "flat" and "quasi-cleavage" on the fracture surface of a lath martensitic steel has been visualized in a transmission electron microscope by using focused-ion beam machining to extract samples perpendicular to the fracture surface.
Total cell numbers were counted under light microscope by using a hemocytometer.
The percentage of cells with internalized bacteria was determined in an Olympus disk scanning confocal microscope by using Slidebook software and by flow cytometry using CellQuest software.
Coverslips were mounted onto slides using Slowfade Gold mounting medium (Invitrogen) and analyzed on an Olympus disk scanning confocal microscope by using Slidebook software.
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Interfacial fracture between an individual fiber and the pyrocarbon matrix in carbon/carbon composites has been studied in a 300 kV high-resolution transmission electron microscope (TEM) by using an integrated TEM-atomic force microscope piezodriven holder.
Thereafter, cells were washed in PBS and mounted on the microscope slides by using fluorescent mounting medium (Dako) and examined under the fluorescence microscopy.
Another study also reported that significantly more canal orifices were found using a surgical microscope than by using a magnifying loupe [ 19].
Biological materials may be killed, dyed so that their structure can be seen, and mounted on glass slides for photographing by transmitted light using ordinary light microscopes; or, by using ultraviolet, infrared, electron, or X-ray microscopes, sharp photographs can be made of living, unstained specimens.
The stainings were either scanned with a Leica confocal microscope or recorded by using a Zeiss Apotome microscope.
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