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In the present work, we intend to test our prototype iCorr system integrated onto our Tecnai Spirit electron microscope by imaging cryo-vitrified rat hippocampal neuron cells.
We tested the performance of this compact dual-mode microscope by imaging a US-air force resolution test target, various micro-particles as well as a histopathology slide.
The reviewer thought that we should show that there is no shift between the red and green channels on our confocal microscope by imaging the same protein (ideally Sas-4) in both channels.
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The performance of the QCL microscope is demonstrated by imaging SU-8, a photoresist polymer that has a distinctive spectrum with multiple peaks throughout the fingerprint region, on an IR-transparent BaF2 substrate.
The microscope functionality was validated by imaging near field interactions of different systems of dipole-like resonances.
Scattering spectra of individual nanorods were collected by imaging the microscope field of view to a line-imaging spectrometer (Acton Research SpectraPro 2150i).
The images were calibrated by imaging a 1 mm ruler (10 microns per tick mark) using the same microscope optics and camera.
Linescans are images constructed with laser-scanning microscopes by repeatedly imaging a one pixel-wide line.
PLA complexes were detected by imaging under a Zeiss LSM510meta confocal microscope and analyzed by LSM software.
The size and shape of the AgNPs were investigated by imaging the films using a scattering emission microscope (Hitachi SU8010).
Calcium records in the whole islet of Langerhans were obtained by imaging intracellular calcium under an inverted epifluorescence microscope (Zeiss, Axiovert 200).
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