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The number of migrated cells was counted by using light microscope at high magnification field (20×).
The morphological analysis was carried out using transmission electron microscopy (TEM on a JEOL 100S microscope at high resolution (HR) on a JEOL 2010 microscope).
Coverslips were mounted in Vectashield mounting medium (Vectra Laboratories, U.K). Immunofluorescence was observed under a Leitz microscope at high magnification (×40) and at least 5 fields/coverslips, 2 coverslips/experiment from at least 3 different experiments (n = 5 for Olig2 quantification) were taken into pictures for quantification.
Invaded cells were counted under microscope at high power fields.
The shapes of these whiskers were measured under a light microscope at high magnification (Pammer et al., 2013).
Goblet cells were counted on a light microscope at high magnification (Olympus, CX31, Tokyo, Japan) and the data were expressed as number of cells/villus, as goblet cell villus index.
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Both GBM QD-stained biopsies were imaged in a confocal scanning Zeiss 510 Meta microscope and a high-speed Programmable Array optical sectioning (PAM) microscope at higher resolutions.
The c-fos-positive neurons were observed under a light microscope at high-power field (HPF) magnification [ 36], and the average number of c-fos-positive neurons was counted single-blind using the imaging software (Image-Pro Plus, Media Cybernetics, Inc., USA).
Stained sections were also visualized with a FX-1000 confocal microscope at higher magnification.
The description of a liquid state for sols was based on physical macroscopic observations and the presence of a "Maltese-cross pattern" visible in cross-polarized reflected light optical microscopes at high magnification [11],[17], though this may not be definitive evidence of a true liquid state [17].
Cells that invaded the lower surface of the filter were counted in five random fields under a light-microscope at high magnification.
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