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After several washings with PBS at 37°C, the cover slips were quickly mounted on glass slides with Fluoromount-G, and examined using an Olympus BX60 fluorescence microscope associated with a digital SensiCam.
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In order to verify that the emission observed using a wide-field microscope is indeed associated with the PCP complexes, we obtain fluorescence spectra and decay curves for an identically prepared structure.
Moreover, their design principle, assembly process, and structural features are revealed by polyacryalmide gel electrophoresis (PAGE), atomic force microscope (AFM) imaging, and cryogenic transmission electron microscope imaging (cryo-TEM) associated with single particle reconstruction.
Acceptor photobleach method was used to detect FRET under laser confocal scanning microscope (LSM 510, Zeiss) associated with AIMS software.
In addition, the mechanical motions associated with microscope-stage movements or raster scanning mirrors movements hinder the speed of detection.
The folding observed might be due to the electron beam damage associated with scanning electron microscope and transmission electron microscope used while characterizing the samples in these studies.
The fractured fatigue surfaces were examined using scanning electron microscope to study the mechanism/s associated with crack propagation.
The atomic force microscope is clearly a powerful tool for the investigation of forces which govern the mechanics of processes occurring at or below the microscale, and the exceptional ability of atomic force microscopy to determine forces associated with the microscope deformation and flow of fluids is discussed.
Microscope focusing indicated r-GV associated with and/or were within J774A.1 macrophages at 12 h post application and clearly internalized at later times (see Figures 2 and 3).
Examination of cells incubated with DiI-labelled particles for periods up to 24 h with the confocal microscope showed that the fluorescence associated with the cells increased with time in all cases, but that there was clearly more in pCRLP- and less in oxCRLP-treated as compared to CRLP-treated cells (Fig. 3A).
The FT-IR spectro-microscope is composed of the Thermo Nicolet Nexus infrared bench associated with an infrared Thermo Nicolet Continuum microscope.
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CEO of Professional Science Editing for Scientists @ prosciediting.com