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The TMA slides were initially assessed by light microscope assessment of staining quality and specificity.
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Immunostained sections were imaged with a digital Olympus microscope and assessment of levels of mGluR5 and arrestin immunoreactivity was performed utilizing the Image-Pro programograMedia CyberneticsicSilver Springing, MD).
Immunostained sections were imaged with a digital Olympus microscope and assessment of levels of mTor, Atg7, LC3 and Cathepsin D immunoreactivity was performed utilizing the Image-Pro Plus program (Media Cybernetics, Silver Spring, MD).
After de-fatting the tissue in acetone, the mammary epithelia was stained with iron/hematoxylin and inspected under a microscope for assessment of overall development.
Scanning electron microscope assessments and microanalysis (Energy dispersive X-ray) were performed with a XL SIRION 200 FEG SEM (FEI company, Eindhoven, The Netherlands) operating with an electron accelerating voltage of 5 kV.
a Fluorescence microscope images for assessment of live and dead cell ratio dependent on titration of C PFA. Green fluorescence represents live cells and red fluorescence represents dead cell, scale bar 50 μm.
A transparent microfluidic system was prepared to obtain clear microscope images for assessment of pattern transfer fidelity.
The lines written at a power of <5% were found to be too close to the resolution of the microscope for accurate assessment of their size.
DAPI is a fluorescent stain that allows examination of nuclei in a fluorescence microscope for morphological assessment of changes during apoptosis [ 23].
A specular microscope is used for assessment of the properties of corneal endothelium.
Nevertheless, the high-throughput data generated with the WSA systems can be used as a 'gold standard' to provide bench-mark criteria to translate into diagnostic practice using a conventional microscope for the future assessment of mild MCDs.
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