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The MODS technique requires a centrifuge, class I cabinet, standard NALC/NaOH decontamination solution, MODS media (7H9 supplemented with OADC, glycerol and PANTA), inverted microscope and tissue culture plates.
Tumor cell-rich areas in the H/E section were marked under a microscope, and tissue was scraped from the corresponding area of another deparaffinized unstained section.
Images were acquired on an Axioplan (Zeiss, Cambridge, UK) epifluorescent microscope and tissue area was quantified using ImageJ™ software available at the National Institutes of Health website.
Tumour cell-rich area in the H/E section was marked under a microscope, and tissue was scraped from the corresponding area of another deparaffinised unstained section.
Fish from other classes were dissected under a binocular microscope, and tissue samples (sonic muscles and white epaxial muscles) were fixed for 2 days in the 2.5% glutaraldehyde solution.
Template slides were analysed by a consultant gastrointestinal histopathologist (MJA) using a light microscope and tissue regions corresponding to normal/tumour regions were identified and marked on the slide.
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The larvae were manually removed from the infested fruits and visually inspected under a binocular microscope, and tissues were stored at −80°C until molecular analysis.
Briefly, tumour cell-rich areas in H and E-stained sections were marked under a microscope, and tissues scratched from the same areas were sequentially deparaffinised and unstained.
There are abundant new opportunities - with specific techniques that include slide scanners, automated microscopes, and tissue microarrays - and also potential pitfalls.
A sidestream dark-field video microscope and a tissue oxygen monitor were used to investigate microcirculation.
After a tissue sample has been taken, it will be sent to a laboratory so that it can be examined under a microscope and the tissue cells can be tested.
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