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Following sterilisation, all the files were visually inspected under a light microscope and scored using an established scale.
These three factors are judged by sight with a microscope and scored qualitatively to stratify breast cancer patients into three groups that predict survival rates.
A series of non-overlapping field images were taken for each prepared FISH slide using a fluorescence microscope and scored using custom MATLAB software.
A pathologist reviewed sections stained for immunohistochemistry with EGFR, using a transmitted light microscope and scored the percent positive staining tumour cells.
The slides were examined with a microscope and scored independently by two pathologists who were given no clinical or pathological information.
Growth cones were photographed under a fluorescence microscope and scored in a blinded manner as collapsed when no filopodia were present at the tip of the neurite.
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The spirochetes were then visualized under a dark-field microscope (Axiostar, Zeiss) and scored for presence or absence of spirochetes.
Sections were examined under an Olympus-CX31 microscope (Olympus, Tokyo, Japan) and scored by two separate investigators in five randomly selected representative fields at × 40 magnification.
The tissue sections were evaluated under a light microscope (400 × ) and scored as follows: 1, no detectable immunostaining; 2, immunostaining <25%; 3, immunostaining between 25% and <50%; 4, immunostaining 50 75%; 5, immunostaining >75%.
Stained cells were imaged on a microscope and scoring was performed analogous to the Hercep Test system [ 24].
The clones were examined using a Zeiss Axiovert fluorescence microscope and individually scored for the expression of each antigen to identify the cell type of origin.
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