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Cell aggregation was followed using a microscope and representative images were taken.
These sections were examined under an Olympus (Olympus, Japan) fluorescence microscope, and representative images were captured.
All slides were observed under Nikon E400 light microscope and representative photographs were taken.
All slides were observed under a Nikon Light Microscope and representative photographs taken.
Photographs were taken on a Zeiss Axiovert 35 microscope and representative pictures are shown.
Immunostaining was evaluated at 20× magnification using an Olympus BH-2 light microscope and representative photographs were taken.
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The fluorescent signals from stained cells were observed on an inverted fluorescent microscope (ApoTome fluorescence microscope, Zeiss) and representative pictures were taken.
The rostrocaudal localization of pSer9GSK3β immunolabeling was first analyzed at the light microscope, and then representative brain areas were selected for the subsequent analysis of subcellular distribution using electron microscopy.
Microscopic observation was performed using a Zeiss LSM 780 confocal laser scanning microscope (Carl Zeiss, Germany) and representative photographs were taken.
Slides were examined by using an Axioplan 2 microscope (Carl Zeiss MicroImaging GmbH, Germany) and representative micrographs were acquired by using Axiocam MRm (Carl Zeiss).
The immunostained sections were rinsed in PBS, mounted in Vectashield (Vector Laboratories), examined under a LeitzOrthoplan fluorescence microscope (Ploem system) and representative fields were recorded with a digital camera (Leica DC 300 F).
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