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The FRET signals were directly visualized under the microscope and quantified as FRET concentration (FRETc) with the Olympus FRET analysis program19,20.
Healing was studied by means of permeability tests on cracked specimens and physical closing of the crack was observed by optic microscope and quantified through crack geometrical parameters.
The ZsGreen1 GFP expression was observed under a fluorescence microscope and quantified by flow cytometry.
For all experiments, three non-overlapping images were acquired with an Olympus FluoView FV1000 laser scanning confocal microscope and quantified using ImagePro software.
Images were taken at random locations within each grid (e.g. starting at the top corner of the section before progressing across the section in a direction dictated by the loading position of the grid in the microscope) and quantified independently by 3 investigators, with each of the investigators blind to the genotype of the images being assessed.
MSCs were observed under fluorescence microscope and quantified by flow cytometer.
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So Dr. Petraco is systematically making marks in jeweler's wax and other materials, creating images of them under a stereo microscope and quantifying the details, assembling a database that can eventually be mined to determine probabilities that a mark matches a certain tool.
Using NCS instead of ionizing radiation allowed us to treat cells directly on the microscope and quantify DSBs before and immediately after damage without a significant time delay in image acquisition.
Autophagic flux was determined by evaluating patterns of GFP and RFP puncta under a Zeiss confocal laser-scanning microscope and quantifying the LC3 puncta (puncta/cell were counted) using ImageJ software.
Tyrp1 signals were quantitatively measured by capturing images of the transfected cells at random (28 cells and 30 cells were examined in Fig. 1C and other figures, respectively, for each construct) with the confocal microscope and quantifying the fluorescent signals of Tyrp1 with MetaMorph software (Molecular Devices, Sunnyvale, CA).
Images were taken with an AxioImager M1 microscope (Carl Zeiss) and quantified by counting the number of positively stained cells in 15 randomly selected fields at x200 or x400 magnifications.
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