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We then performed time-lapse imaging on a spinning disk confocal microscope and observed MT depolymerization during perfusion with colchicine.
We further investigated whether E2F1 levels might be altered by indirect immunofluorescence microscope and observed no changes in the nuclear intensity of the E2F1 signal between HGPS and normal fibroblast cells (data not shown).
Tissue sections were excited by epi-illumination with an X-Cite Hg-halide lamp (EXFO, Mississauga, Ontario) at 365 nm through a 10X NA 0.3 objective of an Olympus IX71 inverted fluorescence microscope and observed with a 410 nm or 510 nm longpass emission filter on a Canon EOS 40D camera attached to the camera port of the microscope.
The samples were examined with a Leica DM5000B fluorescence microscope and observed with blue excitation wavelengths (filter BP 450-490).
Effects on cell growth, survival or morphology were initially analysed by light microscopy (Nikon Eclipse TS100 microscope) and observed changes documented by digital imaging.
In a test phase, we photographed hair strands with an electronic microscope and observed that the washing procedure effectively removed all particulates from the surface of the hair strand without compromising its structural integrity.
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In the mid-1600s, Antonie van Leeuwenhoek built one of the first microscopes and observed single-cell organisms making what he called "pleasing and nimble" movements.
After washed thrice with PBS, the cover glasses were mounted onto glass microscope slides and observed under Olympus microscope (Olympus Corporation, Tokyo, Japan) equipped with a Metamorph image acquisition system (DP2-BSW software, Olympus Corporation).
We observed the ash obtained by this temperature condition using Scanning Electron Microscope (SEM) and, Transmission Electron Microscope (TEM) and observed fiber shaped carbon.
The cover slips were mounted onto microscope slides and observed on a confocal microscope (Leica Microsystems, Groot Bijgaarden, Belgium).
The resulting pellet was transferred unto a microscope slide and observed under a light microscope at 10X and 40X magnification for the presence of paragonimus eggs.
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