Sections were mounted on frozen microscope slides and individual regions were then immediately collected by punching them out of the sections with cannulae made of 21G or 18G stainless steel needles (o.d. 0.819 and 1.270, respectively; i.d. 0.514 and 0.838 mm, respectively).
Large-scale analysis is time consuming, however, since it requires separate hybridizations and individual microscope analysis.
Sections were covered and visualized on an apotome microscope as individual stains and merged images.
Under a dissection microscope, the cochlea was exposed and individual samples of the TM were isolated from the organ of Corti.
Even with low magnification microscope objectives, the morphological structures and individual cell types inside developing zebrafish embryos can be clearly observed from reconstructed amplitude images.
Differential colony counts were scored after 10 to 12 days by morphologic characteristics using an inverted microscope and confirmed by staining individual colonies with May-Grünwald-Giemsa (MGG).
To unambiguously assess whether our precursor cells indeed had the ability to self-renew, we aspirated single cells under the microscope and placed them into individual wells of a multiwell plate.
Fluorescence was detected by a fluorescent microscope, and its intensity in individual cells was analyzed.
Most are publicly held companies that are under the microscope of countless organizations and institutional and individual investors.
Images of the stained samples were acquired with a wide field fluorescent microscope, and then signaling activities in individual cells were quantified with the following image analysis.
