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After washing the cells were incubated with 0.2 μg ml−1 4′,6′-diamino-2-phenylindole (DAPI; Sigma) for 5 min. The cells were washed, mounted and analysed using an Axiovert microscope and imaging system from Zeiss.
Sections (1 μm thick) were cut with a micrometre (Ultracut UCT, Leica Microsystems,Welzlar, Germany), stained with toluidine, and imaged with a microscope and imaging system (Optiphot 2 with DS-L1, Nikon, Tokyo, Japan).
Confocal microscopy images were acquired using an LSM 510 Meta microscope and imaging software (Zeiss).
Ophthalmologists would have made a detailed examination of the eye in vivo with a slit-lamp microscope and imaging modalities.
We conclude that microscope and imaging improvements to reduce the experimental B-factor will be critical for obtaining an atomic resolution structure.
Examples which highlight the importance of being able to detect phase shifts of waves passing through transparent objects include imaging of unstained cells under the optical microscope and imaging of soft matter (e.g., DNA, viruses, proteins and other macromolecules, polymers, etc). in the transmission electron microscope (TEM).
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Dr. Svoboda, 38, of Huntington, decided to build his own, developing microscopes and imaging methods to do groundbreaking research using live mice.
Microscopes and imaging techniques are now sufficiently advanced to allow large scale collection and analysis of 3D data [1] [6].
A BX51 fluorescence microscope (Olympus) and imaging software (cellSens; Olympus) was used to analyze fluorescence digital images.
The method was thoroughly validated using a variety of cell lines, microscope models and imaging conditions, demonstrating consistently high segmentation performance in all cases and very short processing times (<1 s per 1,208 × 960 pixels image).
We estimated the potential illumination biases of the SP5 confocal microscope by exciting and imaging VectaShield media alone.
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