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The nanocomposites are characterized using laser scanning confocal microscopy, transmission electron microscope, and fluorescence spectroscopy, among others.
Several methods have been used in the past to measure CFU including agar plating, imaging using microscope and fluorescence techniques, membrane filtration and flow cytometry.
Images were captured by Zeiss Discovery V12 stereo microscope, and fluorescence with Nikon SMZ 1500 Microscope.
Differential interference contrast microscopy was performed on a Zeiss Axiophot 2 microscope and fluorescence microscopy was performed using a Leica TCS SP2 confocal microscope.
Cells were then analyzed on an inverted microscope, and fluorescence recorded with a dual excitation fluorescence photomultiplier system (IonOptix) as previously described [42].
Epifluorescence intensity images of both donor and acceptor were acquired with the mercury lamp source of the TE 2000-E microscope and fluorescence detected by a cooled CCD camera (Hamamatsu ORCA-ER).
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Dye loading was performed by incubating the cardiomyocytes with 10 μM DCFH-DA for 30 min at 37°C, and the fluorescence intensity of the cells was determined using fluorescent microscope and fluorescence-activated cell sorting (FACS) analysis.
EGFP-positive cells were photographed and quantified by fluorescence microscope and fluorescence-activated cell sorting.
This paper first reviews the fundamental concepts of microscopic imaging and introduces the technical features and associated clinical applications of optical microscopes, electron microscopes, scanning tunnel microscopes, and fluorescence microscopes.
E. coli cells were conveniently visualized using a fluorescence microscope, and the fluorescence intensity in a single bacterial cell factory could be distinguished under 1000 times magnification (Fig. 4c).
Beads were imaged with an Olympus IX70 fluorescence microscope, and the fluorescence intensities of the beads at 509 nm were measured using a Tecan Infinite M1000 fluorescent microplate reader with an excitation wavelength of 488 nm.
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