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For the purposes of this paper, we show some preliminary data where we have cropped an image taken by the iPhone 4 microscope and explored automated image analysis methods to count cells.
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Tissue blocks were cut serially into semithin (1.5 μm) with a diamond knife in a Leica ultramicrotome (EM UC6), placed on slides, stained with 1% toluidine blue and explored with the light microscope for amyloid plaques.
In the early part of the past century, Machu Picchu and much of ancient Egypt were uncovered and explored, and, on adjacent pages, bugs were placed under microscopes and rodents spied on at night.
With a confocal microscope we explored the mixing sections of fluorescent proteins (B-phycoerythrin, BPE; Allophycocyanin alpha subunit, ApcA) inside a micromixer to confirm the numerical results.
Combined with the MTT (3- 4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide) assay, real-time cell electronic sensing (RT-CES) study and fluorescence microscope image, we explored the nanoparticles' cytotoxicity for human cancer cells in vitro and compared the distinction of different size ZnO nanoparticles affecting the cell proliferation.
The robotic microscope then uses machine vision to generate a digital, 3-D spatial map which researchers can review and explore on a screen, something like a topographical map of the Earth.
"I appreciate the fact they were coming to the subject matter and exploring maybe the opposite end of the spectrum," shared Edward, who considers being under the microscope "a badge of honor".
Gledhill's microscope work includes experimenting with liquids and crystals to create abstract images, exposing the delicate beauty of butterflies' wings using a macro lens, and exploring the individualism of snowflakes and their complex and intricate structures.
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