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Fluorescent images were collected with an Axiocam HrC camera mounted on a Zeiss Axioplan microscope and data was recorded using the Axiovision software program.
Parasites were visualized on a Leica SP5 confocal microscope and data acquired and analysed with the LAS AF Lite software (Leica, UK).
To locate FITC-labeled conidia phagocytosed by macrophages, live cell imaging was done using a Nikon A1 Confocal on a Nikon Ti-E live cell microscope and data was analyzed with NIS-Elements imaging software.
Apoptotic (fluorescent green) and total cells were counted under a fluorescence microscope and data were recorded.
The slides were immediately viewed using a Bruker Vertex 70 V FT-IR spectrometer equipped with a Hyperion microscope and data analyzed using OPUS software (version 6, Bruker).
These parameters can be exceedingly useful in assessing the performance of fluorophores and the quality of the microscope and data, and can in fact contain novel information.
Similar(51)
Therefore, the selection of appropriate microscopes and data analysis tools requires the consideration of biological questions and sample properties.
The microscope, camera, and data were controlled by SlideBook™ software (SlideBook™ (Intelligent Imaging Innovations, Denver, CO, USA).
Every journalist is under a daily microscope already, and data about how one uses his desk is unlikely to be a tipping point performance metric.
Modern oscilloscopes have enough computing power to eliminate the need for a dedicated microscope controller and data acquisition system.
The microscope, camera, and data were controlled by SlideBook™ software (SlideBook™ version 4.0.8.1 (Intelligent Imaging Innovations, Denver, CO, USA).
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