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Protoplast release and cell wall degradation were examined with a light microscope and counted using a hemacytometer.
Migration of the cells into the wounded area was photographed with an Olympus U-RLF-T microscope and counted.
Cells migrating to the bottom of the filter were stained with DAPI Vector Labss, Burlingame, CA), photographed with an epifluorescence microscope, and counted.
EYFPBax clusters were visualized using a Zeiss LSM 510 META confocal microscope and counted individually in the SVZ-OB system, the dentate SGZ, the cerebellum, the striatum, and the frontal cortex.
Cells were photographed under the microscope and counted.
The cells were photographed under a fluorescence microscope and counted.
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The brain pieces were then sent to Rush Medical College in Chicago, where they were analyzed in the traditional way, by a pathologist looking under a microscope and counting plaque.
If fusion was taking place, then neutrons flying through the plastic would cause protons within the material to recoil, leaving telltale tracks.Studying CR-39 under a microscope and counting the number of tracks is a standard way to assess how many neutrons bowled past.
Heart rate was calculated by direct observation under a dissecting microscope and counting the number of beats per minute.
However, the best method is to look through the microscope and count the percentage budding yeast cells and calibrate this percentage to the number of transformants obtained.
With a small paintbrush, Villeda swept each brain slice, one after another, onto a microscope slide, and counted the tiny brown tree shapes.
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