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Nematodes were counted using a dissecting microscope and categorized into four feeding groups (bacterial feeders, fungal feeders, plant feeders, and predators; Yeates et al. 1993).
The ER morphology of indicated cells was visualized using calreticulin, an endogenous luminal ER protein, with indirect immunofluorescence and confocal microscope, and categorized as "normal" or "abnormal".
Larval responses were recorded at 6 and after 24 h under the dissecting microscope and categorized as follows: a) swimming (swimming or crawling, elongated body shape), b) metamorphosis (flattened along the oral-aboral axis with clear radial subdivisions of mesenteries), c) settlement (metamorphosis plus attachment to the dish surface or CCA).
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Every EBSLN was dissected and categorized using Cernea's classification.
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Analysis was performed using a fluorescence microscope and cells were categorized as either proliferating (red nuclear fluorescence) or normal (no red fluorescence).
Analysis was performed using a fluorescence microscope and cells were categorized as either apoptotic (green nuclear fluorescence) or normal (no fluorescence).
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