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Images were visualized with an Olympus AX70 microscope and captured using the MicroBrightfield system (Williston, VT).
The slides were visualized either on an inverted IX70 Olympus microscope and captured with a CoolSnap HQ (Photometrics) digital camera, or on an Olympus FV1000 confocal microscope.
Chromogenic imaging was performed on a Zeiss Axioskop microscope and captured using Zeiss Axiovision® software (Carl Zeiss GmbH, Jena, Germany).
For bright-field microscopy, embryos were observed using an Olympus AX70 microscope and captured with a DP50 camera.
Microscopy was conducted with an Olympus BX46 light microscope and captured with the Olympus DP21 digital camera.
Images were collected using a Zeiss Axioplan microscope and captured using an Axiocam MR camera and Axiovision software (Zeiss).
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Finally, the slides were examined with a Nikon A1R laser scanning confocal microscope and the positive cells were counted with NIS elements AR software, which is equipped for Nikon DS series digital cameras in laser confocal microscope to control microscope and capture microscopic image.
All the fluorescent images were captured using an Olympus FluoView FV1000 (Olympus, Japan) laser scanning confocal microscope, and images captured was processed by FV10-ASW1.7 FV10-ASW1.7
Cells were visualized on a Zeiss Axioskop microscope and images captured using a ZeissAxiocam camera.
The preparations were observed with an epifluorescence microscope and images captured with a Vysis imaging station.
Sections were examined on a Nikon E600 microscope and images captured with Photometrics Coolsnap HQ camera using Elements Software.
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