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Immunostained sections were imaged with a digital Olympus microscope and assessment of levels of mGluR5 and arrestin immunoreactivity was performed utilizing the Image-Pro programograMedia CyberneticsicSilver Springing, MD).
Immunostained sections were imaged with a digital Olympus microscope and assessment of levels of mTor, Atg7, LC3 and Cathepsin D immunoreactivity was performed utilizing the Image-Pro Plus program (Media Cybernetics, Silver Spring, MD).
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It did require cultured cells to be physically moved between tissue culture incubator and microscope for assessment, thus care had to be taken when handling weakly adherent cells.
Scanning electron microscope assessments and microanalysis (Energy dispersive X-ray) were performed with a XL SIRION 200 FEG SEM (FEI company, Eindhoven, The Netherlands) operating with an electron accelerating voltage of 5 kV.
This software allowed for the acquisition of data sets from the microscope and for an observational morphological assessment to be made, but was limited in preparing the data for any quantitative analysis.
After de-fatting the tissue in acetone, the mammary epithelia was stained with iron/hematoxylin and inspected under a microscope for assessment of overall development.
For the elemental analysis of the deterioration products and assessment of laser tests, Scanning Electron Microscope (Carl Zeiss Ultra Plus Field Emission) connected with an energy dispersive X-ray spectroscopy (EDS) detector (Oxford Instruments X-Max) was employed.
The TMA slides were initially assessed by light microscope assessment of staining quality and specificity.
Diagnostic and assessment services.
We report a new microcirculatory assessment device, the Braedius Cytocam, an Incident Dark Field (IDF) video microscope, and compare it with the most commonly used precursor device, the Microvision Microscan which utilises side stream dark field (SDF) imaging.
In cases of discrepant assessments, slides were reevaluated by both pathologists under a multihead microscope, and an agreement was obtained.
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