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Over 130 grains were analyzed for one sample of CoTi, which would be a Herculean task on the TEM, requiring dozens of samples as well as numerous hours on the microscope and analysis of the images generated for each sample.
The slides were then mounted in DPX mounting medium and analysed using an Olympus BX60 microscope and analySIS Soft Imaging System.
Images were obtained using an optical microscope and analysis for each enzyme utilized a blind test.
Images were taken with a Bio-Rad confocal microscope, and analysis was performed using ImageJ.
Alternatively, images can be obtained using an inverted microscope and analysis is performed as described for the migration.
Imaging was performed on a Nikon Eclipse 300 vertical microscope, and analysis and quantitation were performed using Adobe Photoshop.
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An image analysis system that consisted of an Olympus BX51 microscope and software analysis (analySIS, Soft imaging system, Münster, Germany) was used.
The information was assessed with computer image analysis software (Nikon H600L Microscope and image analysis system, Tokyo, Japan).
The collagen type II content was evaluated based on optical density measured using image analysis software (Nikon H600L Microscope and image analysis system, Japan).
The collagen type II content was evaluated based on optical density measured using image analysis software (Nikon H600L Microscope and image analysis system).
The hardened layer was evaluated using surface integrity with optical microscopy, energy dispersive spectroscopy, scanning electron microscope (SEM) and analysis of microhardness.
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