Microscopes and acquisition of images For fluorescence and phase contrast microscopy, a Leica microscope type DM5000B with a mercury lamp (Hg100W; Leica Microsystems, Wetzlar, Germany), camera type DFC300FX (Leica Microsystems DI Cambridge) combined with the Leica Application Suite software 2.8.1 was used.
Microscope adjustment and acquisition were controlled by the Gray Institute Open Microscope control software (67), SPCM and DCC-100 programs (Becker & Hickl GmbH).
This discussion includes criteria for the selection of a fluorescent protein with optimal characteristics, selection of microscope and image acquisition system components, the design of a fluorescence signal quantification technique, and possible sources of measurement errors.
Lower power images used in some figures were collected with a Nikon (San Diego, CA) TE2000-U inverted fluorescence microscope and Metamorph acquisition software.
The microscope and image acquisition were controlled by AxioVision software.
The microscope and image acquisition were controlled by MetaMorph software (Molecular Devices, Sunnyvale, CA).
A MediaCybernetics Evolution QEi monochrome digital camera mounted to the Olympus microscope and the InVivo acquisition software version 3.2.0 (MediaCybernetics) were used to capture images and analyze the microscope images.
Images were taken at intervals of 15 sec using a Nikon Diaphot 300 microscope and AQM Kinetic Acquisition Manager software (Kinetic Imaging Ltd., Bromborough, UK).
Live imaging was performed using the wide-field microscope and Volocity 5.0.2 Acquisition (Perkin-Elmer, UK) with image capture every 1, 6 or 10 minutes during the time-lapse experiments.
A description of the microscope and software used for acquisition is described elsewhere (Koschwanez et al., 2013).
