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Single-molecule tdEosFP signals were separated with a 561 nm dichroic (Di01-R561-25×36) and a single band 617 nm emission filter (FF01-617/73), expanded through a 1.5× lens in the tube-lens of the microscope and acquired with an Andor iXon EMCCD camera (512×512 pixels, pixel size 16 µm) at frame rates of 25 or 50 ms for up to 30 minutes.
Parasites were visualized on a Leica SP5 confocal microscope and acquired and analysed with the LAS AF Lite software (Leica).
Wing images were captured by a Nikon Eclipse 90i microscope and acquired with a Nikon Digital Sight camera.
Cells were imaged with a 10x objective on a Leica DMLB microscope and acquired using QCapture Software (QImaging Software).
All images were visualized on a Nikon Eclipse microscope and acquired at the same exposure with QCapture software.
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The images were visualized at 20× in Nikon Inverted Microscope TE2000 and acquired with Image Pro Plus 7.0, (Media Cybernetics, Rockville, MD, USA).
Stained cells were observed using an Olympus laser scanning confocal FV1000 microscope (Olympus, Tokyo, Japan) and acquired images were analysed using Olympus Fluoview software (Tokyo, Japan).
Sections were then viewed with an Eclipse E1000 Fluorescence Microscope (Nikon, Milan, Italy) and acquired using Sigma Scan Pro software (Jandel), as described previously (Cittadini et al, 2009).
Digital images were taken with a Spot Pursuit camera (Diagnostics Instruments), attached to the microscope and were acquired using Spot Advanced software.
Parasites were visualized on a Leica SP5 confocal microscope and data acquired and analysed with the LAS AF Lite software (Leica, UK).
Fluorescent slides were viewed using a Leica DXMRA confocal microscope and images acquired using Volocity software (Improvision, Lexington, MA).
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