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Scanning electron microscope and confocal fluoroscope examinations showed that the scaffold has a regular interconnected porous structure in the scale of 250 μm and high porosity.
The crystal violet staining, [2, 3-bis (2-methoxy-4-nitro-5 sulfophenyl -2H-tetrazolium-5-carboxanilide] (XTT) reduction method, phenol-sulfophenyl -2H-tetrazolium-5-carboxanilidepe and confocal lasulfophenyl -2H-tetrazolium-5-carboxanilidewere conducted.
To examine the surface morphology of the samples, the investigation under Scanning Electron Microscope and Confocal Microscope was performed and the coatings with the lowest amount of cracks were chosen for further evaluation, involving wettability and Surface Free Energy studies.
The nanoparticles were analyzed using microscopy (Phase contrast microscope and Confocal laser scanning microscope), and Malvern zetasizer Nano- ZS.
Wide field microscopy utilized a Zeiss Automated Upright Fluorescent microscope, and confocal images were collected using a Leica TCS SP5 Laser Scanning microscope.
In each experiment, coverslips were viewed with a 63× objective Zeiss LSM510 fluorescence microscope and confocal images were acquired using identical photomultiplier settings and corrected for background fluorescence using unlabelled specimens.
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Subsequent instruments, such as phase-contrast microscopes, interference microscopes, and confocal microscopes, solved specific problems that had arisen during the study of specimens such as living cells.
Images are acquired with both in-house and remote electron microscopes and confocal microscopy.
At least 15 distinct types of collagen in articular cartilage are revealed by electron microscopes and confocal microscopes, and type II collagen represents 90%to95%5% of the collagens in ECM of articular cartilage [ 5, 6].
Images were captured at the indicated magnifications using a Leica TCS SL confocal microscope and Leica Confocal software (version 2.61 Build 1537) (Leica Microsystems Heidelberg GmbH).
The specimens were analyzed with a DM-IRE2 confocal laser scanning microscope and Leica confocal software (Leica Microsystems).
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