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Atomic force microscope analysis was applied to test the layers of GS.
The microscope analysis was implemented for samples taken from the field to determine the rock microstructures.
Figure 5shows clearly differentiated PC12 cells after incubation on the CNT array coated with the releasing film, as described in section "Cell Culture and In Vitro Testing" The microscope analysis was carried out up to three days of incubation, and, specifically, after 8 (Fig. 5a), 24 (Fig. 5b), 48 (Fig. 5c) and finally after 72 h (Fig. 5d).
Microscope analysis was performed using a Leica DM 4000 microscope together with a Leica DFC 290 camera.
Transmission electron microscope analysis was performed to further evaluate the effects of KOR activation on the thoracic aortal ultrastructure in DM rats.
To determine the concentration of intracellular Ca2+ in 16HBE cells, laser scanning confocal microscope analysis was performed with a Leica Sp2 confocal microscope.
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Vickers hardness test and transmission electron microscope analysis were performed before and after uniaxial tensile deformation to correlate the microstructure with its mechanical properties.
Scanning confocal microscope analysis were done as published using a FV1000 instrument (Olympus, Tokyo, Japan) coupled to an inverted Olympus microscope with a 63X oil immersion objective [ 87].
The hydrolysis of riboprobes, the whole in situ hybridisation protocol and the microscope analysis were realised as previously described [ 21, 25, 27].
Therefore immunfluorescence stainings with subsequent confocal laser microscope analysis were performed to deliver precise data about the nuclear localisation of CT45.
Scanning electron microscope (SEM) analysis was performed with PEM 106I (SELMI, Ukraine) microscope to observe the morphology of OSP and CNF films.
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