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Second, the researchers compared 22 genetic markers known as microsatellites with a population from Argentina and found that the European populations had lost less than 30% of their genetic variation.
TRF output data were evaluated for regions containing microsatellites with a period size of 2 4 bp and number of repeats less than 30.
Only mononucleotide microsatellites with a minimum repeat number greater than or equal to 12 were counted.
In general, the microsatellites with a higher GC% tend to have shorter lengths.
An ABI3730 Genetic Analyzer (Applied Biosystems) was used to visualise the microsatellites, with a LIZ500 size standard.
The filters were screened for microsatellites with a radiolabeled (CA 10 probe, following the procedures previously described.
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Each N2 mouse was genotyped using a total of 112 microsatellites with an average interval space between markers of 15 cM based on Kosambi's distances created by the MapManager QTX programme.
SSR-couples containing two microsatellites with an identical motif were not considered [partially standardized: see Additional file 1].
In future studies of tree peony SSRs, attention should focus on Class I microsatellites, with an emphasis on evaluation of polymorphism and its implications.
He for the 22 microsatellites with an otherwise orthologous STR region disrupted by a mutation in either the chimpanzee (16) or human (6) ePCR fragment did not differ significantly between species (ARD = 0.925 with SD = 0.253; P = 0.235, Wilcoxon signed-rank test).
FedSat is a research microsatellite with a complement four scientific and engineering payloads.
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