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quinquefasciatus transcripts (CpipJ1.2 Gene Build) indicated that C99TGT1 and C177GAA1 were within CPIJ005634 and CPIJ008257, respectfully, while no other microsatellites were within coding regions [24].
Additionally, BLASTn analysis indicated that none of the previously reported microsatellites were within these 16 supercontigs, but that two of them are located in other supercontigs with known genetic marker loci (CxpGT4, gb-AY423738, supercontig 3.5, chromosome 2-36.1 2-36.1i4, gb-AY958079, superCxqTri43.208, chromosome 3-26.3) [24].
Although a small number of polymorphic tri-nucleotide microsatellite loci contained within coding regions have been identified in previous studies [ 20], BLAST analyses against the annotated Ae. aegypti genome assembly at VectorBase [ 24] indicated that none of our polymorphic tri-nucleotide microsatellites were within putative coding regions.
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We then wanted to know whether higher segmental duplications with higher frequency of paired microsatellites is a within-genome feature or are they also correlated across genomes.
In this way, classical microsatellites were sometimes recognized within longer minisatellites.
Thorough analysis of the microsatellite distribution and motif type among the 147 annotated genes revealed that the microsatellites were more frequent within the CDS regions of the kinase genes and that trinucleotides were the most abundant motif (Table S3).
The 13 mapped microsatellites were randomly distributed both within and across linkage groups (χ1= 0.27, P > 0.5).
Pentanucleotide microsatellites were fairly evenly distributed within the CDSs, introns, 3′UTRs, and intergenic regions.
Those containing microsatellites were analyzed for redundancy within the dataset and previously discovered salmonid microsatellites using Vector NTI Suite 6.0 (InforMax, Bethesda, MD).
Within each family, microsatellites were genotyped for the 96 least and the 96 most resistant animals.
In our comparative survey microsatellites were found with highly variable abundancies within the EST datasets of 24 phylogenetically well distributed plant species.
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