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Thirty-eight clones containing microsatellites were sequenced.
Clones putatively containing microsatellites were sequenced and those containing perfect, imperfect or compound repeats with at least 10 repeats in length were chosen for primer design.
Microsatellites were sequenced from several homozygous cats (from the Persian and Korat breeds and the Hawaii and Texas random-bred populations) to determine the repeat lengths of the alleles.
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A total of 4300 clones collected from libraries enriched for CA-, GA-, CAA- GACA- and GATA-microsatellites were sequenced in both directions.
The microsatellite was sequenced in 115 A. marginale strains collected from infected cattle in the USA, Canada, Mexico, Puerto Rico, Argentina, Brazil, Italy, Israel, South Africa and China [ 11].
Simple sequence repeats (SSRs), also known as microsatellites, are sequences with repetitive 2 5 base pairs of DNA.
Simple Sequence Repeats (SSRs) or microsatellites are sequences of 2 6 nucleotides that repeat from 3 to 100 times.
Microsatellites are sequences made up of a simple sequence motif, not more than six bases long, that is tandemly repeated and arranged head to tail without interruption by any other base or motif.
In total, 109 positive clones containing microsatellite repeats were sequenced with an ABI 3730xl DNA Analyzer using a BigDye Terminator version 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA).
864 clones collected from the microsatellite-enriched library were sequenced in both directions.
A total of 51 (26.5%) unique microsatellites were obtained from 192 sequenced clones.
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