Sentence examples for microsatellites were run from inspiring English sources

The microsatellites were run in 4% polyacrilamide (29 1 acrylamide:bisacrylamide) gels at 150 constant watts (1800-2000 volts) and 45°C constant temperature in Biorad Sequi-Gen GT vertical gel rigs for 1.5 hrs.

Microsatellites were run on 4% high resolution MetaPhor agarose gels for 2 h at 78 V/cm, stained with ethidium bromide (0.1  μL/mL), and photographed under UV light using an Alpha Imager 2200.

The annealing temperatures for the multiplex PCRs (two microsatellite loci were run in the same multiplex PCR) were 59°C for the microsatellites Gac1125 and Gac5017; 57°C for Gac4174 and Gac2111; Gand188 and Gac1097; Gac7033 and Gac5196.

Single microsatellite marker amplifications were run in a T1 thermocycler (Biometra) in a final volume of 12.5 μL including 50 to 100 ng of genomic DNA, 5 pmol of the forward and the reverse primer, 1U DNA polymerase (FirePol), 3 mM MgCl2, 100 μM dNTPs, and 1× PCR Buffer.

Negative controls were run, and comparisons to microsatellite readings were conducted to test for possible contaminations between neighbouring DNA wells.

Microsatellites were amplified by seminested PCR as described previously (34 ), and products were run with GeneScan-500 LIZ Size Standards Applied Biosystemss) in an ABI 3730 DNA analyzer (Applied Biosystems) and analyzed by using Genemapper software (Applied Biosystems).

Fifteen microsatellite loci were tested with gradient PCR 45-64°C 45-64°Che products were run in 2% androse gel and in ABI377.

MIcroSAtellite (MISA) identification tools (http://pgrc.ipk-gatersleben.de/misa/) and SciRoKo [ 63] were run with default parameters.

PCR products were run on 1 % agarose gel to confirm the presence of target microsatellite fragments.

Genotypes were run on an ABI 3700 sequencer and alleles were visualized using GENEMAPPER Applied Biosystemss, Inc .. We used the computer program CONVERT to translate our microsatellite genotype files into the correct input format for various analysis programs [70].

Both SCAR and CAPS markers were evaluated on 1.5% agarose gels run in 0.5X TBE; while the microsatellite marker was run on 4% polyacrylamide silver stained gels as described in Blair et al. [ 53].