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On the other hand, 9 tetrameric, 2 pentameric and 1 hexameric microsatellites were present.
In 2008, a finishing-level sequence for 181662 (136.6 kb) resulted in a single contig with no Sp185/333 genes, although GA microsatellites were present.
About 33% of the microsatellites were present in the 5'-UTRs, ~ 20% in the 3'-UTRs and ~ 47% in the CDSs, similar to the relative frequencies in both the A. thaliana and rice genomes [ 15].
When comparing our results with those of Hanai et al. (2007) we can observe some similarities, as these authors found that AG motif SSR were four times more likely than AC motif microsatellites, that AT microsatellites were fairly common (27% of all dinucleotide SSR) and that no CG motif microsatellites were present in a set of 3,126 common bean unigenes.
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Rarely but significantly, microsatellites are present in the coding regions (exons) of critical growth regulatory genes and are targeted for frameshift mutation when DNA MMR is defective [15].
It is now known that microsatellites are present within most eukaryote genomes, principally in intergenic regions, but also within introns and exons [ 34].
Since microsatellites are present at a relatively low frequency in avian genomes, in this case their isolation is most efficient using enrichment protocols.
In cucumber genomic DNA, microsatellites are present at a higher density than in coding regions (1.2-1.5 1.2-1.5.3 fold higher compandd to clustered and bulk ESTs, respectively).
High-frequency instability of microsatellites is present in about 15% of the cases of sporadic colorectal carcinoma, and it appears to be associated with different prognostic features as compared with tumours harbouring microsatellite stable or low-frequency microsatellite unstable lesions.
2 Somatic hypermethylation of Msh2 and microsatellite instability (MSI) are frequent events in LS CRCs. 3 In fact, MSI is found in virtually all Lynch tumours as a result of an accumulation of frameshift mutations at repetitive sequences due to polymerase slippage at microsatellites and MMR deficiency. 4 5 Microsatellites are present throughout the genome mostly in non-coding regions.
If altered GA microsatellites are present in the other A2 genes throughout the genome, this may restrict recombination or gene conversion to within the A type element pattern category and maintain the sequence diversity for all of the A type genes so that they share a similar element pattern and individual element sequences.
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