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All microsatellites were polymorphic and useful for T. cruzi genetic variability studies.
All the microsatellites were polymorphic and a total of 148 alleles were detected across these loci.
This showed that most microsatellites were polymorphic.
For the SA-RH11-LG2 group, all 10 genotyped microsatellites were polymorphic (2 18 alleles, mean = 7.1).
Primers were designed by routine techniques and after verification that the microsatellites were polymorphic, they were registered in the Genome DataBase with accession numbers GDB 11524532 for DXS1012 and GDB 11524534 for DXS1019.
All these microsatellites were polymorphic, having 2 to 4 alleles (Table 6).
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Previous studies have estimated that less than 10% of passerine microsatellites are polymorphic in species from different taxonomic families [ 26, 57, 58].
Genotyping of the microsatellites using genomic DNA from sea bream caught at different geographic locations and a family panel, revealed both microsatellites are polymorphic and the locus sbPAC1B scores more alleles (8 alleles) than sbPAC1A (4 alleles) (Additional file 1).
There was large variation in the taxonomic range over which a given microsatellite was polymorphic.
Sequencing the OAS1 promoter regions of 13 horses established that this promoter microsatellite is polymorphic in length.
In silico comparison of the sequences included in the two contigs indicated that the one microsatellite was polymorphic while the other microsatellite was not polymorphic between the two sequenced ESTs.
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