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For fine-resolution mapping, microsatellites were obtained from DOGSET [35].
A total of 51 (26.5%) unique microsatellites were obtained from 192 sequenced clones.
A total of 19 (9.9%) unique microsatellites were obtained from the 192 sequences clones (Table 1).
A total of 14 (7.3%) unique microsatellites were obtained from the cDNA library enriched for GA-microsatellites.
Genotypes of the reference population using the same set of 30 autosomal microsatellites were obtained from a previous study by Li et al. [ 3].
Primer pairs for all 246 microsatellites were obtained from the publicly available primer sequence files provided by the Mammalian Genotyping Service [ 76] (Marshfield Clinic, Marshfield, WI) for the Screening Set from which their genotypes were obtained with one exception.
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Template material for the polymerase chain reaction (PCR) amplification of microsatellites was obtained with three different protocols.
Multilocus genotype information from six microsatellites was obtained from 892 of the individuals utilized in the Pan I analyses, representing the same sampling years and sites (SW, FAX, BRE; Table 1).
Chromosomal locations of each microsatellite were obtained using a BLASTN search on the ENSEMBL webpage http://www.ensembl.org/Gallus_gallus/blastview.org/Gallus_gallus/blastview
Valid genotypes for the D6S1276 microsatellite were obtained from 93.32% of them with a similar distribution of the genotype call rate between the three sample collections and between cases and controls.
Microsatellite loci were obtained from microsatellite-enriched genomic libraries (GL) or using sequence information retrieved from GenBank (GB).
More suggestions(15)
microsatellites were encountered
microsatellites were searched
microsatellites were sequenced
microsatellites were scored
microsatellites were employed
microsatellites were genotyped
microsatellites were analyzed
microsatellites were assigned
microsatellites were analysed
microsatellites were omitted
microsatellites were detected
microsatellites were scattered
microsatellites were performed
microsatellites were developed
microsatellites were amplified
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