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Exact(5)

As predicted and confirming the above analysis, Pfdhfr-ts and its flanking microsatellites were in strong linkage disequilibrium (Fisher's exact test, p = 0.00024 for each pair of loci) While there was no influence of blood group or gender on Pfcrt and Pfdhfr-ts allele distribution, there was an influence of age.

Twenty-nine of 41 microsatellites were in HWE (Table 2).

However, whereas Loots and Ovcharenko (2007) observed that >75% of ECRs shared between human and nonmammalian vertebrates were in coding regions, we found that at most 35% of conserved microsatellites were in exonic regions (human fugu comparison).

Indeed, when the data were analyzed for each of the eight subpopulations (Appendix 1), all microsatellites were in HWE for five of the populations, with only BYU4012 or BYU8677 violating HWE in the remaining three populations (note that the sample sizes for these populations are too small for definitive conclusions).

Exact tests for Hardy-Weinberg equilibrium revealed that the majority of these microsatellites were in HWE, but 6 loci (Locus SL_eSSR02, Locus SL_eSSR06, Locus SL_eSSR11, Locus SL_eSSR16, Locus SL_eSSR17 and Locus SL_eSSR24) showed significant departure from HWE (p < 0.002) after Bonferroni correction (see Additional file 1).

Similar(55)

Though not directly comparable, microsatellites are in Hardy-Weinberg equilibrium in these F. heteroclitus populations [ 66].

This interval on mutation rates in microsatellites is in line with those observed in many model species (Ellegren 2000).

Microsatellites are, in general, specific for particular lineages, and their presence in one species does not mean that the intron cannot be used in other taxa.

The microsatellite is in strong linkage disequilibrium with the SINE element and diagnostic SNP [ 16].

A shorter GT-microsatellite is in the same relative position in the human OAS1 promoter and the flanking regions were well conserved between the two sequences.

Field collection, DNA extraction, the genetic analysis and genotyping of microsatellites were described in detail in [ 34].

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