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Further analysis using JoinMap 4.0 allowed mapping of 290 microsatellite markers, of which 161 microsatellites were from BES with significant similarity to zebrafish chromosome 7, and 129 microsatellites were from BES with significant similarity to zebrafish chromosome 13.
As shown in Table 1, these 106 mapped BAC end sequence-derived microsatellites were from 46 BAC contigs of the physical map [ 71] that included 1645 BAC end sequences (BESs) [ 9, 48].
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Microsatellites were determined from single genotype infections from which we had obtained reliable unambiguous gene sequence at all positions.
Microsatellites were sequenced from several homozygous cats (from the Persian and Korat breeds and the Hawaii and Texas random-bred populations) to determine the repeat lengths of the alleles.
Recently, gene specific microsatellites were developed from E. grandis, E. globulus and E. gomphocephala (Acuna et al. [2012b]; Bradbury et al. [2013a]).
On the basis of the amplification results, the nucleotide sequences of three representative homoeologous microsatellites were amplified from multiple accessions of T. urartu and T. monococcum and compared.
For fine-resolution mapping, microsatellites were obtained from DOGSET [35].
Microsatellites were identified from a partial genomic DNA library enriched with CA- and GA-repeats.
Crabs used for developing microsatellites were collected from three populations along the Portuguese Coast: Esposende, Aveiro and Sado (Figure 1).
In the present study, microsatellites were isolated from whale shark DNA with relative ease, and moderate levels of genetic variation were found.
Both OAS1 microsatellites were omitted from the haplotype reconstruction.
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