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More than 40% of different length categories of microsatellites were distributed in intergenic regions.
The microsatellites were distributed in 9 autosomal linkage groups (LG1 - LG9) and one Z-chromosome linkage group (LGZ) covering in total 999 cM in females and 822.9 cM in males.
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Microsatellites are distributed throughout the genomes and are found in both coding and non-coding regions (3).
Microsatellites are distributed non-randomly across plant genomes and are associated with non-repetitive DNA (Zhang et al., 2006).
In addition, the 19 microsatellites are distributed across five of the six chromosomes (Michel et al. 2010) of the Rhagoletis genome (the small sixth dot chromosome is highly heterochromatic and currently does not contain a marker).
However, although microsatellites are distributed ubiquitously throughout the Coffea genome, only a few of them are suitable for designing informative markers with properties such as strong and specific amplified fragment after PCR and easy scoring of allele sizes, high heterozygosity and/or known position along a linkage map.
In the case of trimeric repeats, it is worth to note that this kind of microsatellite was distributed homogenously along ESTs.
The microsatellite markers were distributed across 32 PCR multiplexes that were subsequently combined into 16 multiplexes for capillary electrophoresis.
To evaluate the consequences of genetic differentiation (or lack thereof) on phenotypic divergence among populations, we used data from microsatellite loci and selected populations that were distributed around the island at various distances from one another (see Figure 1).
Condoms were distributed.
In the first case, we expected shared polymorphism at neutral microsatellites to be distributed evenly across the species distribution range, whereas in the other case a higher proximity among species may be expected in sympatric zones.
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