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Finally, primers to amplify the microsatellites were designed using Primer3 [41].
Primers flanking the microsatellites were designed using the PRIMER 3 program [ 45].
Microsatellites were designed and characterized in the African timber forest tree Terminalia superba (Combretaceae).
Primers for BESs containing microsatellites were designed using Primer3 software [ 52].
Specific primers in the flanking regions of the microsatellites were designed using the PRIMER3 software [ 62].
Primers for amplification of selected microsatellites were designed using primer3 [ 47] (See Table 3).
Similar(50)
However, "neutral" markers such as microsatellites are designed to reconstruct the evolutionary and demographic history of populations and are theoretically "blind" to the effect of natural and anthropogenic selection that is sometimes very effective and rapid in changing morphological and production traits [ 46].
Two multiplex PCR maps for 14 microsatellites loci were designed based on information content reported in Ascaris spp. literature.
Primers for each gene and some microsatellite markers were designed according to the giant panda DNA sequences published in NCBI, and the other microsatellite markers were designed according to the sequences from our laboratory (A204, A066 and B043).
Accordingly, microsatellite markers were designed, and their ability to detect polymorphisms was determined.
The microsatellite markers were designed for the giant liver fluke, Fascioloides magna, veterinary important liver parasite of free-living and domestic ruminants.
More suggestions(15)
microsatellites were sequenced
microsatellites were scored
microsatellites were found
microsatellites were employed
microsatellites were selected
microsatellites were genotyped
microsatellites were analyzed
microsatellites were obtained
microsatellites were assigned
microsatellites were analysed
microsatellites were omitted
microsatellites were detected
microsatellites were performed
microsatellites were scattered
microsatellites were amplified
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