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Crabs used for developing microsatellites were collected from three populations along the Portuguese Coast: Esposende, Aveiro and Sado (Figure 1).
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Similarly, mouse genomic microsatellites are collected in the Mouse Microsatellite Database of Japan (MMDBJ) [ 11].
Microsatellites are collected from both chromosomes, though it is impossible to distinguish the two chromosomes with inexpensive technology.
The contigs harboring two or more BAC-anchored microsatellite markers were collected for assembly assessment.
In addition, for testing transferability of microsatellite primers, leaves were collected from 23 species of this genus (52 individuals) from a living collection in Cartmel, Cumbria, United Kingdom (Pigott, 2002).
For IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Ampliflied Polymorphism) analyses, leaves were collected from 10 unrelated individuals of five Eucalyptus species: Eucalyptus brassiana S.T. Blake, Eucalyptus grandis W. Hill ex Maid., Eucalyptus saligna Sm., Eucalyptus tereticornis Sm. and Eucalyptus urophylla S.T. Blake.
Each sire was determined to be a unique MLG across the six microsatellite loci indicating that no clones were collected (Table 2).
Data were collected from seven microsatellite loci developed from a genomic DNA library from Acropora millepora (van Oppen et al. 2007).
Comparison of mtDNA clade and microsatellite assignment for each individual where both data types were collected.
Only the microsatellites of 2-6 nucleotide motifs with at least 5 repeat units were collected.
A minimum of 30 individuals per age class were collected, totalling 454 samples which were genotyped at six microsatellite loci.
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